Molecules that serve similar functions for different organisms

Determination of agrA Gene Transcription In Methicillin-Resistant Staphylococcus Aureus

Differential functions of FANCI and FANCD2 ubiquitination stabilize ID2 complex on DNA

The Fanconi anaemia (FA) pathway is a dedicated pathway for the repair of DNA interstrand crosslinks and is additionally activated in response to other forms of replication stress. A key step in the FA pathway is the monoubiquitination of each of the two subunits (FANCI and FANCD2) of the ID2 complex on specific lysine residues.

However, the molecular function of these modifications has been unknown for nearly two decades. Here, we find that ubiquitination of FANCD2 acts to increase ID2’s affinity for double-stranded DNA via promoting a large-scale conformational change in the complex. The resulting complex encircles DNA, by forming a secondary “Arm” ID2 interface.

Ubiquitination of FANCI, on the other hand, largely protects the ubiquitin on FANCD2 from USP1-UAF1 deubiquitination, with key hydrophobic residues of FANCI’s ubiquitin being important for this protection. In effect, both of these post-translational modifications function to stabilize a conformation in which the ID2 complex encircles DNA.

Keywords: DNA repair; Fanconi anaemia; deubiquitination; protein-DNA interactions; ubiquitin
cdna-clone
cdna-clone

Recombinant Cryptococcus neoformans var. neoformans serotype D Cofilin (COF1)

MBS1039978-01mgEColi 0.1mg(E-Coli)
EUR 750

Recombinant Cryptococcus neoformans var. neoformans serotype D Cofilin (COF1)

MBS1039978-01mgYeast 0.1mg(Yeast)
EUR 950

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1)

MBS1214026-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1060

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1)

MBS1214026-002mgEColi 0.02mg(E-Coli)
EUR 645

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1)

MBS1214026-002mgYeast 0.02mg(Yeast)
EUR 810

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1)

MBS1214026-01mgEColi 0.1mg(E-Coli)
EUR 745

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1)

MBS1214026-01mgYeast 0.1mg(Yeast)
EUR 950

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1)

MBS1167282-005mgBaculovirus 0.05mg(Baculovirus)
EUR 985

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1)

MBS1167282-005mgEColi 0.05mg(E-Coli)
EUR 645

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1)

MBS1167282-005mgYeast 0.05mg(Yeast)
EUR 810

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1)

MBS1167282-02mgEColi 0.2mg(E-Coli)
EUR 830

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1)

MBS1167282-05mgEColi 0.5mg(E-Coli)
EUR 910

Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5)

MBS1197398-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1230

Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5)

MBS1197398-005mgEColi 0.05mg(E-Coli)
EUR 1010

Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5)

MBS1197398-005mgYeast 0.05mg(Yeast)
EUR 1115

Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5)

MBS1197398-02mgEColi 0.2mg(E-Coli)
EUR 1310

Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5)

MBS1197398-05mgEColi 0.5mg(E-Coli)
EUR 1445

Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5)

MBS1144887-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1320

Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5)

MBS1144887-002mgEColi 0.02mg(E-Coli)
EUR 1010

Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5)

MBS1144887-002mgYeast 0.02mg(Yeast)
EUR 1115

Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5)

MBS1144887-01mgEColi 0.1mg(E-Coli)
EUR 1180

Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5)

MBS1144887-01mgYeast 0.1mg(Yeast)
EUR 1270

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SUS1 (SUS1)

MBS1188036-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1025

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SUS1 (SUS1)

MBS1188036-002mgEColi 0.02mg(E-Coli)
EUR 595

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SUS1 (SUS1)

MBS1188036-002mgYeast 0.02mg(Yeast)
EUR 775

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SUS1 (SUS1)

MBS1188036-01mgEColi 0.1mg(E-Coli)
EUR 695

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SUS1 (SUS1)

MBS1188036-01mgYeast 0.1mg(Yeast)
EUR 910

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein BTN1 (BTN1)

MBS1165009-1mgEColi 1mg(E-Coli)
EUR 2320

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein BTN1 (BTN1)

MBS1165009-1mgYeast 1mg(Yeast)
EUR 2795

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein BFR2 (BFR2)

MBS1048802-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1235

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein BFR2 (BFR2)

MBS1048802-005mgEColi 0.05mg(E-Coli)
EUR 1020

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein BFR2 (BFR2)

MBS1048802-005mgYeast 0.05mg(Yeast)
EUR 1125

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein BFR2 (BFR2)

MBS1048802-02mgEColi 0.2mg(E-Coli)
EUR 1330

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein BFR2 (BFR2)

MBS1048802-05mgEColi 0.5mg(E-Coli)
EUR 1460

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein GET1 (GET1)

MBS1047662-INQUIRE INQUIRE Ask for price

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein PNS1 (PNS1)

MBS1012490-1mgEColi 1mg(E-Coli)
EUR 2405

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein PNS1 (PNS1)

MBS1012490-1mgYeast 1mg(Yeast)
EUR 2880

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SYM1 (SYM1)

MBS1137240-INQUIRE INQUIRE Ask for price

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein YOP1 (YOP1)

MBS1142629-INQUIRE INQUIRE Ask for price

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A (HTA1)

MBS1153408-005mgBaculovirus 0.05mg(Baculovirus)
EUR 980

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A (HTA1)

MBS1153408-005mgEColi 0.05mg(E-Coli)
EUR 635

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A (HTA1)

MBS1153408-005mgYeast 0.05mg(Yeast)
EUR 805

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A (HTA1)

MBS1153408-02mgEColi 0.2mg(E-Coli)
EUR 815

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A (HTA1)

MBS1153408-05mgEColi 0.5mg(E-Coli)
EUR 900

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein GET1 (GET1)

MBS1109967-INQUIRE INQUIRE Ask for price

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SEY1 (SEY1)

MBS1116432-1mgEColi 1mg(E-Coli)
EUR 3070

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SEY1 (SEY1)

MBS1116432-1mgYeast 1mg(Yeast)
EUR 3565

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1)

MBS1125191-005mgBaculovirus 0.05mg(Baculovirus)
EUR 985

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1)

MBS1125191-005mgEColi 0.05mg(E-Coli)
EUR 645

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1)

MBS1125191-005mgYeast 0.05mg(Yeast)
EUR 815

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1)

MBS1125191-02mgEColi 0.2mg(E-Coli)
EUR 830

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1)

MBS1125191-05mgEColi 0.5mg(E-Coli)
EUR 910

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1)

MBS1140841-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1060

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1)

MBS1140841-002mgEColi 0.02mg(E-Coli)
EUR 645

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1)

MBS1140841-002mgYeast 0.02mg(Yeast)
EUR 815

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1)

MBS1140841-01mgEColi 0.1mg(E-Coli)
EUR 750

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1)

MBS1140841-01mgYeast 0.1mg(Yeast)
EUR 950

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SUS1 (SUS1)

MBS1140978-005mgBaculovirus 0.05mg(Baculovirus)
EUR 955

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SUS1 (SUS1)

MBS1140978-005mgEColi 0.05mg(E-Coli)
EUR 595

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SUS1 (SUS1)

MBS1140978-005mgYeast 0.05mg(Yeast)
EUR 775

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SUS1 (SUS1)

MBS1140978-02mgEColi 0.2mg(E-Coli)
EUR 770

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SUS1 (SUS1)

MBS1140978-05mgEColi 0.5mg(E-Coli)
EUR 845

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A (HTA1)

MBS1067999-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1050

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A (HTA1)

MBS1067999-002mgEColi 0.02mg(E-Coli)
EUR 635

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A (HTA1)

MBS1067999-002mgYeast 0.02mg(Yeast)
EUR 805

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A (HTA1)

MBS1067999-01mgEColi 0.1mg(E-Coli)
EUR 735

Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A (HTA1)

MBS1067999-01mgYeast 0.1mg(Yeast)
EUR 940

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein YOP1 (YOP1)

MBS1066890-INQUIRE INQUIRE Ask for price

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SYM1 (SYM1)

MBS1064595-INQUIRE INQUIRE Ask for price

Recombinant Cryptococcus neoformans var. neoformans serotype D ATPase GET3 (GET3)

MBS1103927-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1120

Recombinant Cryptococcus neoformans var. neoformans serotype D ATPase GET3 (GET3)

MBS1103927-005mgEColi 0.05mg(E-Coli)
EUR 855

Recombinant Cryptococcus neoformans var. neoformans serotype D ATPase GET3 (GET3)

MBS1103927-005mgYeast 0.05mg(Yeast)
EUR 990

Recombinant Cryptococcus neoformans var. neoformans serotype D ATPase GET3 (GET3)

MBS1103927-02mgEColi 0.2mg(E-Coli)
EUR 1140

Recombinant Cryptococcus neoformans var. neoformans serotype D ATPase GET3 (GET3)

MBS1103927-05mgEColi 0.5mg(E-Coli)
EUR 1215

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein RAI1 (RAI1)

MBS1102545-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1170

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein RAI1 (RAI1)

MBS1102545-005mgEColi 0.05mg(E-Coli)
EUR 925

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein RAI1 (RAI1)

MBS1102545-005mgYeast 0.05mg(Yeast)
EUR 1050

Recombinant Cryptococcus neoformans var. neoformans serotype D Protein RAI1 (RAI1)

MBS1102545-02mgEColi 0.2mg(E-Coli)
EUR 1205

Fluorometric determination of agrA gene transcription in methicillin-resistant Staphylococcus aureus with a graphene oxide-based assay using strand-displacement polymerization recycling and hybridization chain reaction

A graphene oxide (GO)-based fluorescent bioassay was developed to quantify agrA gene transcription (its mRNA) in methicillin-resistant Staphylococcus aureus (MRSA). This method is based on the use of Klenow fragment (KF)-assisted target recycling amplification and hybridization chain reaction (HCR). A triple complex was designed that contained a capture probe (CP), a trigger probe (TP), and a help probe (HP), which were partially complementary to one another.
In the absence of the target, all the oligonucleotides labeled with carboxyfluorescein (FAM) are adsorbed onto the surface of GO by π-stacking interactions. This adsorption quenches the FAM signal. On the contrary, the target RNA causes the triple complex to disintegrate and initiates strand-displacement polymerization reaction (SDPR) and HCR in the presence of the appropriate raw materials, including the primer, KF, dNTPs, hairpin 1 (H1), and hairpin 2 (H2), generating double-stranded DNA (dsDNA) products.
These dsDNA products are repelled by GO and produce strong fluorescence, measured at excitation/emission wavelengths of 480/514 nm. The fluorescent signal is greatly amplified by SYBR Green I (SGI) due to the synergistic effect of dsDNA-SGI. The target was assayed with this method at concentrations in the range 10 fM to 100 pM, and the detection limit (LOD) was 10 fM.
This method also displayed good applicability in the analysis of real samples. It provides a new way of monitoring biofilm formation and studying the mechanisms of drug actions. Graphical abstract Schematic representation of the graphene oxide-based fluorescent bioassay for agrA gene transcription in methicillin-resistant Staphylococcus aureus by using strand-displacement polymerization recycling and hybridization chain reaction.
Keywords: Biofilms; Drug-resistant bacteria; Dual fluorescence; Enzymatic reaction; Fluorescent assay; Quenching

The structure of human CST reveals a decameric assembly bound to telomeric DNA

The CTC1-STN1-TEN1 (CST) complex is essential for telomere maintenance and resolution of stalled replication forks genome-wide. Here, we report the 3.0-angstrom cryo-electron microscopy structure of human CST bound to telomeric single-stranded DNA (ssDNA), which assembles as a decameric supercomplex.
The atomic model of the 134-kilodalton CTC1 subunit, built almost entirely de novo, reveals the overall architecture of CST and the DNA-binding anchor site. The carboxyl-terminal domain of STN1 interacts with CTC1 at two separate docking sites, allowing allosteric mediation of CST decamer assembly. Furthermore, ssDNA appears to staple two monomers to nucleate decamer assembly.
CTC1 has stronger structural similarity to Replication Protein A than the expected similarity to yeast Cdc13. The decameric structure suggests that CST can organize ssDNA analogously to the nucleosome’s organization of double-stranded DNA.

Rye Genomic DNA

PLG-1097 0.1mg
EUR 307

Pea Genomic DNA

PLG-1141 0.1mg
EUR 307

Cat Genomic DNA

GC-130 0.1mg
EUR 177

Genomic DNA Kit

abx098076-100l 100 µl
EUR 300

Genomic DNA Kit

abx098076-1ml 1 ml Ask for price

Genomic DNA Kit

abx098076-200l 200 µl
EUR 550

Clam Genomic DNA

GCL-325 0.025mg
EUR 177

Duck Genomic DNA

GD-220 0.1mg
EUR 177

Goat Genomic DNA

GG-150 0.1mg
EUR 177

Pork Genomic DNA

PCR-705 20µg
EUR 90.1

Corn Genomic DNA

PLG-1002 0.1mg
EUR 307

Rice Genomic DNA

PLG-1004 0.1mg
EUR 307

Pear Genomic DNA

PLG-1033 0.1mg
EUR 307

Crab Genomic DNA

GRA-340 0.025mg
EUR 177

Human Genomic DNA

BIO-35025 500µl @ 200ng/µl Ask for price

Human Genomic DNA 

X11000
  • Ask for price
  • Ask for price
  • 0.2 ml
  • 0.2 ml

Llama genomic DNA

GL-260 0.1mg
EUR 177

Yeast Genomic DNA*

GY-300 0.05mg
EUR 177

Ecoli Genomic DNA*

GE-310 0.05mg
EUR 177

Goose Genomic DNA

GG-140 0.1mg
EUR 177

Human Genomic DNA

PCR-261 20µg
EUR 96

Horse Genomic DNA

PCR-706 20µg
EUR 90.1

Apple Genomic DNA

PLG-1001 0.1mg
EUR 307

Beans Genomic DNA

PLG-1051 0.1mg
EUR 307

Lemon Genomic DNA

PLG-1062 0.1mg
EUR 307

Wheat Genomic DNA

PLG-1084 0.1mg
EUR 307

Onion Genomic DNA

PLG-1092 0.1mg
EUR 307

Maple Genomic DNA

PLG-1094 0.1mg
EUR 307

Lotus Genomic DNA

PLG-1161 0.1mg
EUR 307

Quail Genomic DNA

GQ-200 0.1mg
EUR 177

Camel Genomic DNA

GC-270 0.1mg
EUR 177

Squid Genomic DNA

GSQ-380 0.025mg
EUR 177

Donkey Genomic DNA

GD-160 0.1mg
EUR 177

Ferret Genomic DNA*

GF-180 0.05mg
EUR 177

Gerbil Genomic DNA*

GG-120 0.05mg
EUR 177

Shirmp Genomic DNA

GHR-375 0.025mg
EUR 177

Orange Genomic DNA

PLG-1003 0.1mg
EUR 307

Cotton Genomic DNA

PLG-1022 0.1mg
EUR 307

Banana Genomic DNA

PLG-1031 0.1mg
EUR 307

Barley Genomic DNA

PLG-1041 0.1mg
EUR 307

Pepper Genomic DNA

PLG-1043 0.1mg
EUR 307

Potato Genomic DNA

PLG-1073 0.1mg
EUR 307

Tomato Genomic DNA

PLG-1074 0.1mg
EUR 307

Carrot Genomic DNA

PLG-1081 0.1mg
EUR 307

Radish Genomic DNA

PLG-1083 0.1mg
EUR 307

Cherry Genomic DNA

PLG-1091 0.1mg
EUR 307

Squash Genomic DNA

PLG-1111 0.1mg
EUR 307

Lentil Genomic DNA

PLG-1151 0.1mg
EUR 307

Oyster Genomic DNA

GOY-330 0.025mg
EUR 177

Pigeon Genomic DNA

GP-210 0.1mg
EUR 177

Turkey Genomic DNA

GT-150 0.1mg
EUR 177

Alpaca Genomic DNA

GAP-260 0.1mg
EUR 177

Catfish Genomic DNA

GFC-190 0.025mg
EUR 177

Lobster Genomic DNA

GLB-370 0.025mg
EUR 177

Apricot Genomic DNA

PLG-1011 0.1mg
EUR 307

Soybean Genomic DNA

PLG-1044 0.1mg
EUR 307

Spinach Genomic DNA

PLG-1054 0.1mg
EUR 307

Cabbage Genomic DNA

PLG-1071 0.1mg
EUR 307

Lettuce Genomic DNA

PLG-1072 0.1mg
EUR 307

Ginseng Genomic DNA

PLG-1088 0.05mg
EUR 307

Tobacco Genomic DNA

PLG-1101 0.1mg
EUR 307

Mussels Genomic DNA

GMU-345 0.025mg
EUR 177

Ostrich Genomic DNA

GO-320 0.1mg
EUR 177

Crawfish Genomic DNA

GCF-405 0.025mg
EUR 177

Wildboar Genomic DNA

GW-250 0.1mg
EUR 177

Cucumber Genomic DNA

PLG-1032 0.1mg
EUR 307

Broccoli Genomic DNA

PLG-1061 0.1mg
EUR 307

Rapeseed Genomic DNA

PLG-1093 0.1mg
EUR 307

Flaxseed Genomic DNA

PLG-1095 0.1mg
EUR 307

Chickpea Genomic DNA

PLG-1121 0.1mg
EUR 307

Silkworm Genomic DNA*

GK-390 0.025mg
EUR 177

Starfish Genomic DNA

GSF-390 0.025mg
EUR 177

Alligator Genomic DNA

GL-100 0.025mg
EUR 177

Zebrafish Genomic DNA

GZ-270 0.025mg
EUR 177

Persimmon Genomic DNA

PLG-1053 0.1mg
EUR 307

Sunflower Genomic DNA

PLG-1064 0.1mg
EUR 307

Nectarine Genomic DNA

PLG-1082 0.1mg
EUR 307

Asparagus Genomic DNA

PLG-1131 0.1mg
EUR 307

Jellyfish Genomic DNA

GJF-365 0.025mg
EUR 177

Salmonella Genomic DNA

MBT103-250R 1 unit
EUR 119.62
Description: Salmonella Genomic DNA

Drosophila Genomic DNA*

GD-290 0.025mg
EUR 177

Arabidopsis Genomic DNA

PLG-1021 0.05mg
EUR 307

Dog Skin Genomic DNA

DG-101 0.1mg
EUR 210

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